FastQCFastQC Report
Fri 20 Mar 2020
272_R2_001.fastp-trim.202003184536.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename272_R2_001.fastp-trim.202003184536.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26947532
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3603721.337309850861296No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA2402550.8915658769790124No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2088020.7748464683147979No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA951270.35300820869235816No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT938740.3483584322304543No Hit
CACACACACACACACACACACACACACACACACACACCT523540.19428124252714496No Hit
CACACACACACACACACACACACACACACACACACACACCT500390.18569047436329233No Hit
CACACACACACACACACACACACACACACACACACCT494090.1833525979299329No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT481960.17885125806697252No Hit
CACACACACACACACACACACACACACACACACCT469100.17407902141094034No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT463540.17201575268562627No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT451740.16763687301679428No Hit
CACACACACACACACACACACACACACACACACACACACACCT446220.16558844795137453No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT435360.16155839429005966No Hit
CACACACACACACACACACACACACACACACCT424740.15761740258811086No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT413430.15342035775298457No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT400220.1485182390728769No Hit
CACACACACACACACACACACACACACACACACACACACACACCT386960.14359756581790126No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT337590.12527677859330494No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT320780.11903873052270612No Hit
CACACACACACACACACACACACACACACCT277240.10288140672771072No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT270820.10049899931466823No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT269740.10009822049752087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)