FastQCFastQC Report
Fri 20 Mar 2020
272_R1_001.fastp-trim.202003184536.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename272_R1_001.fastp-trim.202003184536.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26947532
Sequences flagged as poor quality0
Sequence length15-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2470510.9167852551395059No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA2451340.9096714311351407No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT993280.36859776249639487No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA988730.366909296183413No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT788880.29274666043628783No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT596400.22131896902469586No Hit
CACACACACACACACACACACACACACACACACACACCT532490.19760251142850485No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT513350.1904998201690604No Hit
CACACACACACACACACACACACACACACACACACACACCT506560.18798010890199518No Hit
CACACACACACACACACACACACACACACACACACCT503970.18701898192383629No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT487620.18095163594202243No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT487460.18089226130244507No Hit
CACACACACACACACACACACACACACACACACCT467970.17365968801892506No Hit
CACACACACACACACACACACACACACACACACACACACACCT463350.17194524530112812No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT462580.17165950484816198No Hit
CACACACACACACACACACACACACACACACCT434580.16126894292211993No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT434170.1611167954082029No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT433090.16071601659105553TruSeq Adapter, Index 11 (95% over 23bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT419570.15569885954676665No Hit
CACACACACACACACACACACACACACACACACACACACACACCT403740.1498244811435793No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT396770.14723797340698955Illumina PCR Primer Index 11 (95% over 22bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT372510.13823529368106885No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT332190.12327288450756826No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT307950.11427762661159471No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT286860.1064513069323009RNA PCR Primer, Index 11 (96% over 25bp)
CACACACACACACACACACACACACACACACACACACACACACACACCT283450.1051858849263079No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT281620.10450678748614159Illumina PCR Primer Index 11 (95% over 24bp)
CACACACACACACACACACACACACACACCT280250.10399839213476024No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT274840.10199078713404998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)