FastQCFastQC Report
Fri 20 Mar 2020
254_R2_001.fastp-trim.202003184228.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename254_R2_001.fastp-trim.202003184228.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16503023
Sequences flagged as poor quality0
Sequence length15-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA668070.40481674175694965No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT498180.30187196612402467No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG402080.24364021064504365No Hit
GAGCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCG315770.1913407016399359No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG313440.18992883909814584No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT276310.16742993086781738No Hit
GTGCAGCAGTCATCATGGCTGTGGTTGCAATGGTGGCAGTGACAGAGGCA197830.11987500714263077No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG175640.1064289857682438No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA171500.10392035447081424No Hit
GTCCTGTGCTTCGTGCATGAAGGCTATGTGCTTCCTAATTATTGTATGTA168320.1019934347785857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)