FastQCFastQC Report
Fri 20 Mar 2020
254_R1_001.fastp-trim.202003184228.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename254_R1_001.fastp-trim.202003184228.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16503023
Sequences flagged as poor quality0
Sequence length15-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA676560.4099612537654465No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT577580.34998436347086226No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG409230.24797275020461404No Hit
GAGCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCG336440.2038656796394212No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG326720.1979758496367605No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT289420.17537392997634432No Hit
GTGCAGCAGTCATCATGGCTGTGGTTGCAATGGTGGCAGTGACAGAGGCA205850.12473472284441461No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA180160.10916787790939879No Hit
GTCCTGTGCTTCGTGCATGAAGGCTATGTGCTTCCTAATTATTGTATGTA177600.10761664696219596No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG175580.10639262879291872No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC168140.10188436385261052No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA166830.10109056989134658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)