FastQCFastQC Report
Fri 20 Mar 2020
222_R2_001.fastp-trim.202003182800.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename222_R2_001.fastp-trim.202003182800.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51394555
Sequences flagged as poor quality0
Sequence length31-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA539965710.506282231648859No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT48462519.429502794605382No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2767440.5384694935095751No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2573010.5006386376922614No Hit
CACACACACACACACACACACACACACACACACACACACCT1398110.2720346542547163No Hit
CACACACACACACACACACACACACACACACACACACACACCT1381870.26887478644381685No Hit
CACACACACACACACACACACACACACACACACACACACACACCT1363510.26530242357385914No Hit
CACACACACACACACACACACACACACACACACACACCT1341080.2609381480197659No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT1333330.2594302061765103No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1304650.25384984849075937No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT1303520.2536299808413557No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1266230.24637434841103303No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1265100.24615448076162932No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1214020.2362156847160171No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1166580.22698513490388234No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1121910.21829355269249828No Hit
CACACACACACACACACACACACACACACACACACCT1120880.2180931423572011No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1098470.21373275826592916No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1040160.20238719841041528No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACCTGT1034680.2013209375973778No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT960960.18697700563804862No Hit
CACACACACACACACACACACACACACACACACCT948050.18446506638689644No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT925990.18017278289499736No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT925950.18016499996935473No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT884130.17202795121000658No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT851750.16572767290231427No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG846580.164721729763007No Hit
CACACACACACACACACACACACACACACACCT843060.16403683230645738No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT835810.16262617703373441No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT802910.15622472069268817No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT756910.14727435620368734No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT726620.14138073576082136No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT707020.13756710219594276No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT702060.13660201941625918No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA684390.13316391201363648No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT639280.1243867176201837No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT639160.12436336884325587No Hit
CACACACACACACACACACACACACACACCT621930.12101087362270187No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT607280.11816037710609617No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT570670.1110370544116979No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT557780.10852900662336701No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT515590.10031996580182473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)