FastQCFastQC Report
Fri 20 Mar 2020
222_R1_001.fastp-trim.202003182800.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename222_R1_001.fastp-trim.202003182800.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51394555
Sequences flagged as poor quality0
Sequence length31-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA550281610.707001938240344No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT51356469.992587736191119No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2718450.5289373553287892No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2588700.5036914902755749No Hit
CACACACACACACACACACACACACACACACACACACACCT1449520.28203765943688003No Hit
CACACACACACACACACACACACACACACACACACACACACCT1444620.28108425104566037No Hit
CACACACACACACACACACACACACACACACACACACACACACCT1426720.2776013918205927No Hit
CACACACACACACACACACACACACACACACACACACACACACACCT1414100.2751458787803494No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCT1384150.2693184132054456No Hit
CACACACACACACACACACACACACACACACACACACCT1381220.26874831390212445No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1366010.2657888564265222No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1336180.25998473962854624No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1317080.25626839263420026No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1298160.252587068805246No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1254650.24412119143749758No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1215310.23646668406799126No Hit
CACACACACACACACACACACACACACACACACACCT1176170.22885109132669795No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1115010.21695099801914813No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT1108560.21569600125927735No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1078570.20986075275873095No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACCTGT1061740.20658608679460308No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT1059160.20608408809065473No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT1020900.19863971971349884No Hit
CACACACACACACACACACACACACACACACACCT980820.1908412282196042No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT973910.18949672781484342No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT941310.18315364341611676No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT921980.17939254459932574No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT894030.1739542253065524No Hit
CACACACACACACACACACACACACACACACCT875960.1704382886475036No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT866170.16853341759647497No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT839110.16326826839924968No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT802000.1560476591343188No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT792110.1541233307691836No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT722340.14054796271706216No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT722180.14051683101449172No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT718300.1397618872271586No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA669240.13021612892649817No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA667550.1298873003180979No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT664260.12924715468399328No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT648880.12625461977440997No Hit
CACACACACACACACACACACACACACACCT643160.1251416614075168No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCT622130.12104978825091492No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT588370.11448099900855256No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT579060.11266952306523521No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGT530890.10329693486012283No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCTGT519580.10109631263467503No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT518280.10084336755129021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)