FastQCFastQC Report
Fri 20 Mar 2020
221_R2_001.fastp-trim.202003182309.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename221_R2_001.fastp-trim.202003182309.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25272415
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA640810.2535610466985446No Hit
TCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGAC482380.19087214261082686No Hit
GTTAAAAGCTCTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCG419140.16584881183693762No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA402200.15914585131654413No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT379830.15029430309687458No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA374690.14826046501689688No Hit
GCCCCAGAGCCTGCTATGCCCTGTATGCCCCGGTGTGCGGCAGTGACGGC334390.13231422481784982No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA325350.12873720220248044No Hit
TCATAGGGCGCAGCCGTCACCACCATCACCATCATCACCAGGATCAGAAC319070.12625227941215747No Hit
CTTCTGACGTGCATAAAACGAGCAAGCCACAAGAGGACTGAGTGCGCAGG288610.11419961250240629No Hit
CTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCGCACTCAGTCC286700.11344384776840678No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT282570.11180965491426126No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC280630.11104201952998952No Hit
GTCCTCTTGTGGCTTGCTCGTTTTATGCACGTCAGAAGTACTGGTTTTTT271680.10750060886543689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)