FastQCFastQC Report
Fri 20 Mar 2020
221_R1_001.fastp-trim.202003182309.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename221_R1_001.fastp-trim.202003182309.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25272415
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA643610.25466897405728733No Hit
TCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGAC504440.1996010274443499No Hit
GTTAAAAGCTCTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCG435780.17243306585460866No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA427150.16901827545962664No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT406000.160649467017695No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA404560.16007967580462729No Hit
GCCCCAGAGCCTGCTATGCCCTGTATGCCCCGGTGTGCGGCAGTGACGGC357550.14147836682802178No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA341540.13514339646606782No Hit
TCATAGGGCGCAGCCGTCACCACCATCACCATCATCACCAGGATCAGAAC299100.11835038321426743No Hit
CTTCTGACGTGCATAAAACGAGCAAGCCACAAGAGGACTGAGTGCGCAGG290150.11480897254971477No Hit
CTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCGCACTCAGTCC289630.11460321461166255No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT286180.11323808983035456No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC282600.11182152556453351No Hit
GTCCTCTTGTGGCTTGCTCGTTTTATGCACGTCAGAAGTACTGGTTTTTT270960.10721571325890303No Hit
GCCCTGTATGCCCCGGTGTGCGGCAGTGACGGCAGTACCTACGGTAACGA264260.10456460136476867No Hit
GAGATATATTTGTTTAGCGAGTGAGAGGAGGAGATGAGGCCCAGCGTTCT263620.10431136082562746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)