FastQCFastQC Report
Fri 20 Mar 2020
132_R1_001.fastp-trim.202003180140.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename132_R1_001.fastp-trim.202003180140.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26962537
Sequences flagged as poor quality0
Sequence length15-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA601980.2232653403498343No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA468770.17385975214424368No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG429340.1592357573769857No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT406360.15071282053317164No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG362850.1345756150469075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310970.11533410227679984No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC306140.11354272782268227No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT295160.109470410740651No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG288380.10695581057524371No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC284290.10543889100643608No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT281950.10457102015288844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)