FastQCFastQC Report
Fri 20 Mar 2020
127_R2_001.fastp-trim.202003185538.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename127_R2_001.fastp-trim.202003185538.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26948882
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6982052.590849594428444No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6527442.422156139909626No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA888290.32962035308180876No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT762610.2829839100560832No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT513830.19066839210621056No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT511410.18977039566984635No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT467700.173550798879152No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT440200.16334629392046765No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG438560.1627377343520225No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT436500.1619733241623901No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT431190.16000292702309507No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT426510.15826630581558077No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT423750.15724214459063643No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT420790.1561437687841744No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT414720.1538913562351121No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT414350.15375405925930435No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT373960.1387664245218039No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT369660.13717081101917325No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT358950.1331966201789002No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT340740.1264393825317132No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT317130.11767835118354818No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT303800.11273194932539317No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT295470.10964091200518077No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT282890.10497281482771716No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT281390.10441620546633437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)