FastQCFastQC Report
Fri 20 Mar 2020
127_R1_001.fastp-trim.202003185538.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename127_R1_001.fastp-trim.202003185538.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26948882
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7045972.6145685746815026No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT5926262.199074529325558No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT875060.3247110585144126No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA857430.31816904315362693No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT632010.23452178832502218No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT627760.23294472846777095No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT618620.22955312209241183No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT601510.22320406464357226No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT597810.2218310948854947No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG585960.2174338809305707No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT578790.21477328818316097No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT494990.18367737852724278No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT411970.15287090573924367No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT404930.15025855246982045No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT388910.14431396449025233No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT380380.14114871258852224No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT364780.1353599752301413No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT341900.12686982710451586No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT295390.1096112261725737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)