FastQCFastQC Report
Fri 20 Mar 2020
118_R2_001.fastp-trim.202003184931.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename118_R2_001.fastp-trim.202003184931.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28556288
Sequences flagged as poor quality0
Sequence length15-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5537711.9392261347133073No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT5497211.9250436191146414No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA667310.23368233294187254No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG541460.1896114789149066No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG509830.17853510932513358No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA508380.1780273402481443No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT489950.17157342018682542No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT484880.1697979793452146No Hit
ATCCAGAACTAGCTGCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTG477150.16709104488650625No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT472820.16557474136694517No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT467280.1636347133072758No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT458450.16054257472119626No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT457290.16013635945960483No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT456190.15975115533223366No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT449840.15752747696059097No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT426470.14934364018180513No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT393560.13781903306200022No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG393330.13773849038082261No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT390450.1367299559382508No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT389950.13655486315308207No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT358860.12566759377129127No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT336520.11784444812995302No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT332460.11642269471438303No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT331280.11600947574138488No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT329050.1152285619195324No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG326350.11428306087962134No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT316090.11069015692795926No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT311660.10913883485136444No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT307770.10777661298275183No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA306770.10742642741241439No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT299530.10489108388317137No Hit
GTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGGCGCTGGGGCAGG298900.10467046697385878No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG295460.10346582861189803No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT291830.10219465499157314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)