FastQCFastQC Report
Fri 20 Mar 2020
118_R1_001.fastp-trim.202003184931.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename118_R1_001.fastp-trim.202003184931.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28556288
Sequences flagged as poor quality0
Sequence length15-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5732852.007561346908954No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT4770171.6704447020565136No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA694000.24302878581417864No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT639800.22404872790188976No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT635330.22248339840248146No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT622240.21789946928676443No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT608180.21297586016782014No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT604760.2117782255172661No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT583350.20428075245634167No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA532320.18641078280202245No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG531430.18609911764442214No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG530440.18575243392978807No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG520130.18214202069960914No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT501020.1754499744504608No Hit
ATCCAGAACTAGCTGCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTG494700.1732368016459282No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT417760.1462935238641661No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT405530.14201075433893928No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG401110.14046293411804783No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT385800.13510159303618174No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT376490.13184136537634025No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT376480.13183786352063687No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT338890.11867438793165275No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA319830.11199985096102126No Hit
GTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGGCGCTGGGGCAGG309180.1082703746369276No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG303350.10622879276186037No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT297540.10419421459819987No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT291930.10222967354860688No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG290450.10171139890450748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)