FastQCFastQC Report
Fri 20 Mar 2020
113_R2_001.fastp-trim.202003184225.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename113_R2_001.fastp-trim.202003184225.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30861709
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA11076003.5889133683426278No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT10631633.4449258788617314No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT776220.2515155592971212No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT763170.24728701835663086No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT727230.23564151939868266No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT709680.229954860892506No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT701510.22730756744547104No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT699600.22668867754536862No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT683330.22141677248009822No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT681310.22076223970616793No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT679680.22023407712126378No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT678110.2197253561039021No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG664990.21547413333461216No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT642970.20833907804652038No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT616570.19978478832782723No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT566780.18365152752882222No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT552780.17911516176890915No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT550160.178266213319554No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT518740.16808531244980632No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT498160.16141685478273415No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT493030.15975460075785175No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT477470.15471275424183412No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT470450.15243809083936344No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT441860.1431741839053696No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT409220.13259797116225808No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGT381580.12364188904768689No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT380130.12317205116541019No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT377720.12239114820245373No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT372770.12078721888019875No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA352360.11417384565449698No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT329850.10688001756480822No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT329320.10670828371818294No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTCT314280.1018349307875335No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT313010.10142341760788426No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTCT309210.10019211833019358No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTCT308760.10004630657362494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)