FastQCFastQC Report
Fri 20 Mar 2020
113_R1_001.fastp-trim.202003184225.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename113_R1_001.fastp-trim.202003184225.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30861709
Sequences flagged as poor quality0
Sequence length15-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA11229193.6385509305398482No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT9304673.014956171092145No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG995490.3225647678811306No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT985110.3192013766962808No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT983420.3186537725438342No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT978770.31714705105929164No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT960770.3113145807965463No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT934890.3029287846632213No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT926240.3001259586758465No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT756150.24501235495416018No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT664010.21515658773141824No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT641430.20784007781292996No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT634660.20564642094188626No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT624100.202224705054409No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT567470.18387510555556077No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT558810.18106903930692886No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT495610.16059058816217858No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT428320.13878687016328228No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGCT408010.1322058995501513No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT392280.12710896859276327No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG389300.1261433707381532No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT378380.12260500544542105No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT377080.12218377148200056No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG372970.12085202410534038No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG358050.11601755430977591No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT353540.1145561964828325No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA351170.11378825456490435No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT350220.1134804297454817No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT348710.1129911502956625No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCT347690.11266064364744027No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA346730.11234957856676052No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGT335530.10872048595883008No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG333050.10791690116707406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)