WARNING An augustus species is mentioned in the config file, dataset default species (fly) will be ignored INFO ****************** Start a BUSCO 3.0.2 analysis, current time: 02/07/2020 15:02:39 ****************** INFO Configuration loaded from /gscratch/scrubbed/samwhite/outputs/20200207_cbai_busco_megan/config.ini INFO Init tools... INFO Check dependencies... INFO Check input file... INFO To reproduce this run: python /gscratch/srlab/programs/busco-v3/scripts/run_BUSCO.py -i /gscratch/srlab/sam/data/C_bairdi/transcriptomes/20200122.C_bairdi.megan.Trinity.fasta -o 20200207.cbai.megan -l /gscratch/srlab/sam/data/databases/BUSCO/metazoa_odb9/ -m transcriptome -c 28 --long -z INFO Mode is: transcriptome INFO The lineage dataset is: metazoa_odb9 (eukaryota) INFO Temp directory is ./tmp/ INFO ****** Step 1/2, current time: 02/07/2020 15:02:40 ****** INFO Create blast database... INFO [makeblastdb] Building a new DB, current time: 02/07/2020 15:02:40 INFO [makeblastdb] New DB name: /gscratch/scrubbed/samwhite/outputs/20200207_cbai_busco_megan/tmp/20200207.cbai.megan_3008243817 INFO [makeblastdb] New DB title: /gscratch/srlab/sam/data/C_bairdi/transcriptomes/20200122.C_bairdi.megan.Trinity.fasta INFO [makeblastdb] Sequence type: Nucleotide INFO [makeblastdb] Keep MBits: T INFO [makeblastdb] Maximum file size: 1000000000B INFO [makeblastdb] Adding sequences from FASTA; added 19670 sequences in 0.881879 seconds. INFO [makeblastdb] 1 of 1 task(s) completed at 02/07/2020 15:02:41 INFO Running tblastn, writing output to /gscratch/scrubbed/samwhite/outputs/20200207_cbai_busco_megan/run_20200207.cbai.megan/blast_output/tblastn_20200207.cbai.megan.tsv... INFO [tblastn] 1 of 1 task(s) completed at 02/07/2020 15:03:36 INFO ****** Step 2/2, current time: 02/07/2020 15:03:36 ****** INFO Maximum number of candidate transcript per BUSCO limited to: 3 INFO Getting coordinates for candidate transcripts... INFO Extracting candidate transcripts... INFO Translating candidate transcripts... INFO Running HMMER to confirm transcript orthology: INFO [hmmsearch] 319 of 1592 task(s) completed at 02/07/2020 15:03:45 INFO [hmmsearch] 478 of 1592 task(s) completed at 02/07/2020 15:03:45 INFO [hmmsearch] 796 of 1592 task(s) completed at 02/07/2020 15:03:46 INFO [hmmsearch] 956 of 1592 task(s) completed at 02/07/2020 15:03:46 INFO [hmmsearch] 1115 of 1592 task(s) completed at 02/07/2020 15:03:47 INFO [hmmsearch] 1592 of 1592 task(s) completed at 02/07/2020 15:03:48 INFO Results: INFO C:85.5%[S:64.7%,D:20.8%],F:9.3%,M:5.2%,n:978 INFO 836 Complete BUSCOs (C) INFO 633 Complete and single-copy BUSCOs (S) INFO 203 Complete and duplicated BUSCOs (D) INFO 91 Fragmented BUSCOs (F) INFO 51 Missing BUSCOs (M) INFO 978 Total BUSCO groups searched INFO BUSCO analysis done with WARNING(s). Total running time: 73.68021702766418 seconds INFO Results written in /gscratch/scrubbed/samwhite/outputs/20200207_cbai_busco_megan/run_20200207.cbai.megan/