FastQCFastQC Report
Wed 18 Dec 2019
329777_S4_L002_R1_001.fastp-trim.201912181245.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L002_R1_001.fastp-trim.201912181245.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21255544
Sequences flagged as poor quality0
Sequence length23-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG566640.26658456730159436No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA551490.259457015073338No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT533950.2512050503153436No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA378040.17785477520594156No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG362460.17052492281543113No Hit
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC343250.16148728068310086No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT331830.15611456474602578No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA327130.1539033769260387No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC311150.14638533833808254No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG309220.14547733993540696No Hit
GTGATTTTTTTCTTTTCTTTAGAACTAACAACACAAACTCAAGAGAAAAT306960.1444140879198387No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA286720.13489186632908573No Hit
CTTGGAAATTGATGATGCATTAGGTGATAATATAATCTCTAGAGAGCACG280870.1321396431914422No Hit
CTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGAT276910.13027659983672965No Hit
GGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCG266300.12528496094948216No Hit
GTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATT256940.12088140392925253No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG254730.11984167518836499No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA253610.11931475383551698No Hit
GTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCC249310.1172917522129756No Hit
GTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCAC249280.11727763824816717No Hit
GGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCT245960.11571569280936775No Hit
CTCAAGAGAAAATAAGTGCTTCACAATTTACTCATGACTCAATAAAAACG245210.11536284368915706No Hit
CACCTGAATAGCAAACCAATGATGCAAGATATGAAAAGAAAAAAGTTCAA235910.11098751459854427No Hit
GTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAA229960.1081882449115393No Hit
CTCAATAAAAACGTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTAC221260.10409519511709511No Hit
GCTTGTGATAACTAGTACATGTAAAGAGGAAGTAAGAGGAAGTCTTTTGT220040.10352122721488569No Hit
GCACCGTCATGGGGGCAATACGTGGGTTTTCCCAACCTAGTGCCAAGACA219160.10310721758050512No Hit
CGTGTATGGAAAGTAAATTAACACTCTAAGCAATTAAGGAAGTCAATAGT217420.10228860762161628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)