FastQCFastQC Report
Wed 18 Dec 2019
329777_S4_L001_R2_001.fastp-trim.201912180900.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L001_R2_001.fastp-trim.201912180900.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23725687
Sequences flagged as poor quality0
Sequence length15-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC1527810.6439476336343811No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG1454370.6129938408105949No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG1021940.43073146838698495No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT870270.3668049738665102No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC722050.30433259951545344No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG721520.3041092129387023No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG708340.29855405240741817No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC688100.2900232140801655No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA608270.2563761378121527No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC601820.2536575653215016No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG507370.21384839140801276No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACCAACATATC489300.20623217359311874No Hit
TGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTG413290.17419516661414272No Hit
GCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGACC409700.17268203866973378No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGACCAAGTTTTC408250.1720708867144711No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA396780.1672364640062899No Hit
GAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTGCT396500.16711844845630813No Hit
ACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTG391680.1650868950601936No Hit
GACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGT362460.15277112945138321No Hit
GCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACC355660.14990503752325487No Hit
ATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGC344520.14520970457040927No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT342110.14419392787235202No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG336270.14173246068701825No Hit
GCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCC316380.1333491417972428No Hit
ACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCAC315650.13304145839907608No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT304130.12818596148554096No Hit
GGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGC302730.1275958837356322No Hit
GCCTCGGGCTGTGGGGCATAAGGCAATGCTTTTGGCCTTGTGCTCAGTTG282140.11891752597090234No Hit
CATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGG266820.11246038945047197No Hit
ACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAA264200.1113561010899284No Hit
GTTTAAGAGCCGCGGTATTTTGACCGTGCAAAGGTAGCATAATCATTAGC263440.11103577316854935No Hit
GGAGTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGG261500.1102180940008186No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC261350.11015487138475695No Hit
GGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTGC260470.10978396537052859No Hit
TGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGAC258130.10879769255996675No Hit
GGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGA255580.10772290808691862No Hit
GTTTTCATCAAGAACAGGCCTGGATTTCATGGAGTCCAGACCCTGCCTGG253330.10677456884599379No Hit
GAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGG252390.10637837378534075No Hit
CTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCT242150.1020623765288651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)