FastQCFastQC Report
Wed 18 Dec 2019
329777_S4_L001_R1_001.fastp-trim.201912180900.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L001_R1_001.fastp-trim.201912180900.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23725687
Sequences flagged as poor quality0
Sequence length17-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG659370.2779139756838232No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA636840.2684179387513626No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT623260.26269418457724747No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG426960.17995685435789488No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA417370.17591482177101975No Hit
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC400310.16872430290427418No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT376600.15873091472546189No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA364360.15357194925483084No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC362400.15274584040495856No Hit
GTGATTTTTTTCTTTTCTTTAGAACTAACAACACAAACTCAAGAGAAAAT349910.1474815039075581No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG348570.14691671520407396No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA331610.13976834474803615No Hit
CTTGGAAATTGATGATGCATTAGGTGATAATATAATCTCTAGAGAGCACG316740.13350087607579075No Hit
CTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGAT312800.13184022869390463No Hit
GGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCG306740.12928603500501376No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG297050.12520185400743086No Hit
GTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATT295540.12456541300574352No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA288620.12164874298476584No Hit
GTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCAC288620.12164874298476584No Hit
GTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCC288030.12140006736158998No Hit
GGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCT284350.11984900584754406No Hit
CTCAAGAGAAAATAAGTGCTTCACAATTTACTCATGACTCAATAAAAACG282120.11890909628876078No Hit
CACCTGAATAGCAAACCAATGATGCAAGATATGAAAAGAAAAAAGTTCAA263390.11101469896319545No Hit
GTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAA262340.11057214065076387No Hit
CTCAATAAAAACGTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTAC257410.1084942240028708No Hit
GCTTGTGATAACTAGTACATGTAAAGAGGAAGTAAGAGGAAGTCTTTTGT257200.10840571234038449No Hit
GCACCGTCATGGGGGCAATACGTGGGTTTTCCCAACCTAGTGCCAAGACA252790.10654696742817185No Hit
CGTGTATGGAAAGTAAATTAACACTCTAAGCAATTAAGGAAGTCAATAGT243330.10255972777521678No Hit
GGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTG237600.10014462384166156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)