FastQCFastQC Report
Wed 18 Dec 2019
329775_S2_L002_R2_001.fastp-trim.201912185856.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329775_S2_L002_R2_001.fastp-trim.201912185856.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18176860
Sequences flagged as poor quality0
Sequence length15-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC571270.31428420530278606No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA330470.18180807906316052No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG309740.1704034690260034No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT306920.16885204595293138No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG302100.1662003228280352No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT288540.15874028847666757No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC279800.1539319772501961No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT256350.14103095914255817No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC215790.11871687409156478No Hit
GGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGA215110.11834277207394457No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA214500.11800718055813819No Hit
ACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAA197780.10880867212488847No Hit
GAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGG196220.10795043808446564No Hit
GTTTAAGAGCCGCGGTATTTTGACCGTGCAAAGGTAGCATAATCATTAGC196100.10788442008135618No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG193300.1063440000088024No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG188550.10373078738572009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)