FastQCFastQC Report
Wed 18 Dec 2019
329775_S2_L002_R1_001.fastp-trim.201912185856.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329775_S2_L002_R1_001.fastp-trim.201912185856.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18176860
Sequences flagged as poor quality0
Sequence length15-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA441580.24293524844225023No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA268930.14795184646853196No Hit
GTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAATT239540.13178293720697634No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT235580.12960434310436456No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA234390.12894966457352922No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA217760.11980066964261155No Hit
GCCAAGACATGTTGAACGGATTGGAGGGCAAAGACCGCTGTGCTCGCTAG215910.11878289209467421No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT208020.114442208390228No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG204260.11237364429279866No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG199810.10992547667748995No Hit
GACAGCTTTTCCTTTGTCCGACCATTCATACAAGCTTTCAATCAAAAAGC197520.10866563311815133No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC192460.10588187398703627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)