FastQCFastQC Report
Wed 18 Dec 2019
329775_S2_L001_R2_001.fastp-trim.201912185554.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329775_S2_L001_R2_001.fastp-trim.201912185554.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20572225
Sequences flagged as poor quality0
Sequence length15-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC665310.3234020627326407No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA393280.19117037656354624No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG368730.17923681079708198No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT367650.17871183112181593No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG356560.17332106760450072No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT342180.16633106044679172No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC326880.15889384838052276No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT306690.1490796450067992No Hit
GGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGA256580.12472156025903859No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA255360.1241285276629047No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC254520.1237202101376978No Hit
GAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGG232980.11324978216989169No Hit
ACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAA229800.11170400868160833No Hit
GTTTAAGAGCCGCGGTATTTTGACCGTGCAAAGGTAGCATAATCATTAGC228260.11095542655206231No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG225070.10940479214085982No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG225060.10939993121794069No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG206330.10029542259041013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)