FastQCFastQC Report
Wed 18 Dec 2019
329775_S2_L001_R1_001.fastp-trim.201912185554.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329775_S2_L001_R1_001.fastp-trim.201912185554.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20572225
Sequences flagged as poor quality0
Sequence length15-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA505800.24586548124959745No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA315070.15315309841303018No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA280840.13651415926084806No Hit
GTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAATT279550.13588710020428027No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT270410.13144421665619543No Hit
GCCAAGACATGTTGAACGGATTGGAGGGCAAAGACCGCTGTGCTCGCTAG255190.1240458919732795No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA252240.12261191971213614No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT243800.1185093007683904No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG241000.11714824235103398No Hit
GACAGCTTTTCCTTTGTCCGACCATTCATACAAGCTTTCAATCAAAAAGC231620.11258869665289001No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC226820.11025545365170757No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG225110.10942423583253634No Hit
GCACCGTCATGGGGGCAATACGTGGGTTTTCCCAACCTAGTGCCAAGACA211810.1029592083500934No Hit
CTTGGAAATTGATGATGCATTAGGTGATAATATAATCTCTAGAGAGCACG207680.10095164718449269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)