FastQCFastQC Report
Wed 18 Dec 2019
304428_S1_L001_R2_001.fastp-trim.201912183855.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename304428_S1_L001_R2_001.fastp-trim.201912183855.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30868860
Sequences flagged as poor quality0
Sequence length15-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC1491230.4830855431655072No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT1096220.3551216339054957No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG1050010.3401518553001309No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC831240.26928108132273104No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC755020.24458953132703962No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG635410.2058417447226752No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC632180.2047953827902942No Hit
GGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGC552900.1791125425428733No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG490870.15901785812628No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA472020.15291138059520176No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACCAACATATC465140.15068259728412386No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG442750.143429332991241No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG420740.1362991701021677No Hit
GACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGT391110.12670050011565054No Hit
CTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCT375520.12165010304883302No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG370520.12003034773554967No Hit
ATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGC345040.11177607465905771No Hit
TGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTG325170.10533916704406965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)