FastQCFastQC Report
Fri 25 Oct 2019
329777_S4_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22451464
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC1331900.5932352562844009No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG1249310.5564492364506831No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG879790.39186308741380965No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT750620.3343300909018672No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG633580.28219986010711823No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC626380.27899294228652527No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG606230.2700180264413938No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC601920.2680983298015666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG525610.23410945495581045No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA518660.23101388844843257No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC516050.22985138073846767No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG439680.1958357815775399No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACCAACATATC424970.18928387030796745No Hit
TGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTG356080.15859990243843342No Hit
GCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGACC351010.15634169780643256No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGACCAAGTTTTC350150.15595864928897288No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA339670.15129080223899874No Hit
GAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTGCT338210.15064051056982297No Hit
ACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTG335710.14952699743767267No Hit
GACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGT315600.14056989780265555No Hit
GCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACC308930.1375990447660785No Hit
ATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGC298430.13292228961104718No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT296010.1318444088991257No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG295080.13143018201396578No Hit
ACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCAC273270.12171589344908643No Hit
GCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCC272910.12155554755805678No Hit
GGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGC258380.11508380923399918No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT257140.11453150672045263No Hit
GCCTCGGGCTGTGGGGCATAAGGCAATGCTTTTGGCCTTGTGCTCAGTTG248110.1105094972871257No Hit
GTTTAAGAGCCGCGGTATTTTGACCGTGCAAAGGTAGCATAATCATTAGC234820.10459006147661462No Hit
CATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGG229330.10214478663841252No Hit
ACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAA227380.10127624639533529No Hit
GGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTGC225180.10029635483904302No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC224890.10016718731571357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)