FastQCFastQC Report
Fri 25 Oct 2019
329777_S4_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22451464
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAAGGATATCTCGT3877911.7272414841188084TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAAGGATATCGCGT1095630.48799935719113907TruSeq Adapter, Index 9 (97% over 38bp)
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG578870.25783173872314075No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA561650.25016186026888937No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT542810.2417704253050046No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA385100.17152556287643425No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG371230.16534779201926433No Hit
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC351150.15640405454183298No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT336030.14966952711858791No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA332360.14803488984059124No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC318920.14204864324215116No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG313550.13965681703429228No Hit
GTGATTTTTTTCTTTTCTTTAGAACTAACAACACAAACTCAAGAGAAAAT311720.13884172542155826No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA293330.13065072282146056No Hit
CTTGGAAATTGATGATGCATTAGGTGATAATATAATCTCTAGAGAGCACG284900.1268959565398497No Hit
CTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGAT281710.1254751137832259No Hit
GGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCG272860.1215332772954138No Hit
GTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATT262090.11673626272211024No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG259550.11560493337984551No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA257370.11463394992861045No Hit
GTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCAC255190.11366296647737538No Hit
GTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCC255010.11358279353186054No Hit
GGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCT253010.1126919830261403No Hit
CTCAAGAGAAAATAAGTGCTTCACAATTTACTCATGACTCAATAAAAACG249180.11098608090768602No Hit
CACCTGAATAGCAAACCAATGATGCAAGATATGAAAAGAAAAAAGTTCAA239740.10678145532068645No Hit
GTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAA235500.1048929370485595No Hit
GCTTGTGATAACTAGTACATGTAAAGAGGAAGTAAGAGGAAGTCTTTTGT226010.10066604119891692No Hit
CTCAATAAAAACGTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTAC225230.10031862510168602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)