FastQCFastQC Report
Fri 25 Oct 2019
329777_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24737036
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC1531540.6191283385770228No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG1457510.5892015518754955No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG1024390.414111860450864No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT872740.352807021827514No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC723510.2924804734083744No Hit
TGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGG723000.2922743048116193No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG710600.28726157814541725No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC689870.27888143106554886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG618800.2501512307295021No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCA610430.24676764022981573No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC603200.24384489718169955No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG508460.2055460484433139No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACCAACATATC491090.19852418858912604No Hit
TGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTG414240.16745741082318835No Hit
GCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGACC410300.16586465735021771No Hit
TGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGACCAAGTTTTC409010.1653431720760725No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA398670.1611632048399008No Hit
GAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTGCT397590.16072661251736062No Hit
ACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTG392540.1586851391573348No Hit
GACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGT363320.14687289131971995No Hit
GCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCAACC356660.14418057199738885No Hit
ATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGC344990.13946294940105192No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT344410.13922848315376185No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG337170.13630169758414065No Hit
GCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCC317090.12818431440209732No Hit
ACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCAC316060.12776793468708214No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT305000.12329690590255032No Hit
GGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGC303540.12270669776282007No Hit
GCCTCGGGCTGTGGGGCATAAGGCAATGCTTTTGGCCTTGTGCTCAGTTG282350.11414059469372159No Hit
CATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGG267240.1080323446996641No Hit
ACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAA265540.10734511604381383No Hit
GTTTAAGAGCCGCGGTATTTTGACCGTGCAAAGGTAGCATAATCATTAGC263620.10656895191485351No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC262290.10603129655468828No Hit
GGAGTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGG261890.10586959569448821No Hit
GGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGCTGC261520.10572002239880315No Hit
TGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCGAC258790.10461641402793771No Hit
GGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGA256130.10354110330760727No Hit
GTTTTCATCAAGAACAGGCCTGGATTTCATGGAGTCCAGACCCTGCCTGG253950.1026598336195169No Hit
GAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGG252700.1021545184313917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)