FastQCFastQC Report
Fri 25 Oct 2019
329777_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329777_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24737036
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAAGGATATCTCGT4712521.9050463442750374TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAAGGATATCGCGT1275740.5157206384790806TruSeq Adapter, Index 9 (97% over 38bp)
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG670240.2709459613512306No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA644770.26064965907799137No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT631170.25515182983118917No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG435120.1758981957256318No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA423440.17117653060778987No Hit
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC407630.16478530410838227No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT380400.15377751805026277No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC370350.1497147839377361No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA368790.14908415058295585No Hit
GTGATTTTTTTCTTTTCTTTAGAACTAACAACACAAACTCAAGAGAAAAT354610.14335185508886353No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG352420.14246654287926816No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA337720.13652403626691573No Hit
CTTGGAAATTGATGATGCATTAGGTGATAATATAATCTCTAGAGAGCACG320270.12946983624068786No Hit
CTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGAT317720.12843899325691244No Hit
GGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCG313660.12679772952588175No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG301930.12205585180051481No Hit
GTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATT300080.12130798532208951No Hit
GTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCAC294740.11914927883841864No Hit
GTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCC293680.11872077155888847No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA291500.1178395018707981No Hit
GGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCT291250.11773843883317306No Hit
CTCAAGAGAAAATAAGTGCTTCACAATTTACTCATGACTCAATAAAAACG286060.1156403701720772No Hit
GTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAA267050.10795553679106906No Hit
CACCTGAATAGCAAACCAATGATGCAAGATATGAAAAGAAAAAAGTTCAA266580.10776553828033399No Hit
GCTTGTGATAACTAGTACATGTAAAGAGGAAGTAAGAGGAAGTCTTTTGT262210.10599895638264828No Hit
CTCAATAAAAACGTTTGAAAGCAGTGAAAGTTAGTATATAATCTTAGTAC261030.10552193884505807No Hit
GCACCGTCATGGGGGCAATACGTGGGTTTTCCCAACCTAGTGCCAAGACA256740.10378769711941237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)