FastQCFastQC Report
Fri 25 Oct 2019
329776_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329776_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22546152
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACCGAACATCTCGT1147760.5090713484057058TruSeq Adapter, Index 6 (97% over 37bp)
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA383930.17028626437007965No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT380760.16888025947842453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACCGAACATCGCGT349820.1551572969081376TruSeq Adapter, Index 6 (97% over 37bp)
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG316410.1403388037124916No Hit
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC280850.12456671098465051No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC275380.12214057636087967No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG267020.11843262655197215No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA239790.10635517759305446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)