FastQCFastQC Report
Fri 25 Oct 2019
329776_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329776_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25206233
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACCGAACATCTCGT1423520.5647492031038513TruSeq Adapter, Index 6 (97% over 37bp)
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA455150.1805704168488802No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT449650.17838841686498733No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAACCGAACATCGCGT414550.1644632896950528TruSeq Adapter, Index 6 (97% over 37bp)
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG378050.1499827443474001No Hit
GTGAAAGTTAGTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTC330660.13118183903163952No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC318940.1265321954295987No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG318720.126444915430243No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA271000.10751309011544881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)