FastQCFastQC Report
Fri 25 Oct 2019
329775_S2_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329775_S2_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19604689
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACCAACTATCTCGT4833212.4653336760404616TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACCAACTATCGCGT1636790.8348972023988752TruSeq Adapter, Index 5 (97% over 36bp)
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA453230.23118448856801554No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA274350.13994101105097867No Hit
GTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAATT244360.12464365030223126No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA239960.12239928927207158No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT239680.12225646629742508No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA223790.11415126248623479No Hit
GCCAAGACATGTTGAACGGATTGGAGGGCAAAGACCGCTGTGCTCGCTAG223160.11382991079328011No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT212260.10827001642311183No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG210610.10742838103680195No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG204740.10443419938974804No Hit
GACAGCTTTTCCTTTGTCCGACCATTCATACAAGCTTTCAATCAAAAAGC201610.10283764256602082No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC200300.102169435077496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)