FastQCFastQC Report
Fri 25 Oct 2019
329775_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329775_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21879815
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACCAACTATCTCGT5944912.717075075817597TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACCAACTATCGCGT1943570.8882936167421892TruSeq Adapter, Index 5 (97% over 36bp)
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA516330.2359846278407747No Hit
GGGTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAA320210.14634950066990968No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA286260.1308329160918408No Hit
GTCTTATCGTCCCCCTAAAGAATCTAAGCCTTTTCACTTAGAGGTTAATT283710.1296674583400271No Hit
GCCCCAACAAAACACATTAATTAGATTAAACTTTTACTTTTTAATTAAGT274220.12533012733425763No Hit
GCCAAGACATGTTGAACGGATTGGAGGGCAAAGACCGCTGTGCTCGCTAG262610.12002386674658812No Hit
CTTCTCAATCCTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGA257970.11790319068054277No Hit
CTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAGT248300.11348359206876293No Hit
GTTCCGATCCAGCAGTTCTTTGTGTTGTCTAATAATATTCAGCAGTCTTG247280.11301740896803743No Hit
GACAGCTTTTCCTTTGTCCGACCATTCATACAAGCTTTCAATCAAAAAGC235390.10758317654879623No Hit
GTCGTGTGGAGCCAATGCCGCCGCCAAGGTTAGGCTGGGAAATGAATGGC234290.10708043006762169No Hit
CTTTCGTACTAAAAAAATAACTCCTCAATTAGGAGATAGAAACCGACCTG229900.10507401456547964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)