FastQCFastQC Report
Fri 25 Oct 2019
329774_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename329774_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25811020
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGACTAGATCTCGT2314710.8967913705076359TruSeq Adapter, Index 8 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGACTAGATCGCGT521950.2022198270351191TruSeq Adapter, Index 8 (97% over 41bp)
GCTACCTTTGCACGGTCAAAATACCGCGGCTCTTAAACAATCCTATGTCA321960.12473741835851508No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA273290.10588113139271521No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG264740.10256859279486048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)