FastQCFastQC Report
Tue 24 Sep 2019
EPI-214_S30_L004_R2_001_val_2.20bp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-214_S30_L004_R2_001_val_2.20bp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28640958
Sequences flagged as poor quality0
Sequence length1-79
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCAATAAACACATCAATTATCTTCTACACCCTCTAACCTACCCAATA432460.1509935526597958No Hit
ATAACCAACTTCTACATAAACCAAACTCAAAAACTAAATATCACTAAACA316510.11050957164212175No Hit
ACTAAATATCACTAAACAAACCAAAAAATATAAAAAACAACTAAAATATC294770.10291904342026549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)