FastQCFastQC Report
Tue 24 Sep 2019
EPI-184_S18_L003_R2_001_val_2.20bp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-184_S18_L003_R2_001_val_2.20bp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20675534
Sequences flagged as poor quality0
Sequence length1-79
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAACAATCTATAAACTATTTAAAACAATTAAAACAAAAAAACAACAT549320.2656860035634388No Hit
ATTTCAATAAACACATCAATTATCTTCTACACCCTCTAACCTACCCAATA415270.2008509187719166No Hit
ATAACCAACTTCTACATAAACCAAACTCAAAAACTAAATATCACTAAACA257910.12474163907930988No Hit
ACTAAATATCACTAAACAAACCAAAAAATATAAAAAACAACTAAAATATC232830.11261135988071698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)