21440 reads; of these: 21440 (100.00%) were paired; of these: 20636 (96.25%) aligned concordantly 0 times 667 (3.11%) aligned concordantly exactly 1 time 137 (0.64%) aligned concordantly >1 times ---- 20636 pairs aligned concordantly 0 times; of these: 935 (4.53%) aligned discordantly 1 time ---- 19701 pairs aligned 0 times concordantly or discordantly; of these: 39402 mates make up the pairs; of these: 31345 (79.55%) aligned 0 times 5843 (14.83%) aligned exactly 1 time 2214 (5.62%) aligned >1 times 26.90% overall alignment rate