[22 Apr 19 03:04:53] WARNING =============================================== It seems you have more than 20,000 splits in your samples to be merged. This is the soft limit for anvi'o to attempt to create a hierarchical clustering of your splits (which becomes the center tree in all anvi'o displays). If you want a hierarchical clustering to be done anyway, please see the flag `--enforce- hierarchical-clustering`. But more importantly, please take a look at the anvi'o tutorial to make sure you know your better options to analyze large metagenomic datasets with anvi'o. [22 Apr 19 03:04:53] WARNING =============================================== Anvi'o just set the normalization values for each sample based on how many mapped reads they contained. This information will only be used to calculate the normalized coverage table. Here are those values: MG1: 0.92, MG2: 0.92, MG3: 0.90, MG5: 1.00, MG6: 0.99, MG7: 0.83 [22 Apr 19 03:05:35] profiler_version .............................: 31 [22 Apr 19 03:05:35] output_dir ...................................: /gscratch/scrubbed/samwhite/outputs/20190401_metagenomics_pgen_anvio/SAMPLES-MERGED [22 Apr 19 03:05:35] sample_id ....................................: SAMPLES_MERGED [22 Apr 19 03:05:35] description ..................................: None [22 Apr 19 03:05:35] profile_db ...................................: /gscratch/scrubbed/samwhite/outputs/20190401_metagenomics_pgen_anvio/SAMPLES-MERGED/PROFILE.db [22 Apr 19 03:05:35] merged .......................................: True [22 Apr 19 03:05:35] contigs_db_hash ..............................: hash8fc73e85 [22 Apr 19 03:05:35] num_runs_processed ...........................: 6 [22 Apr 19 03:05:35] merged_sample_ids ............................: MG1, MG2, MG3, MG5, MG6, MG7 [22 Apr 19 03:05:35] Common layer additional data keys ............: default [22 Apr 19 03:05:35] total_reads_mapped ...........................: 143703308, 144858782, 148323108, 132765105, 134500522, 160565140 [22 Apr 19 03:05:35] cmd_line .....................................: /usr/lusers/samwhite/.conda/envs/anvio54/bin/anvi-merge MG1.bam-ANVIO_PROFILE/PROFILE.db MG2.bam-ANVIO_PROFILE/PROFILE.db MG3.bam-ANVIO_PROFILE/PROFILE.db MG5.bam-ANVIO_PROFILE/PROFILE.db MG6.bam-ANVIO_PROFILE/PROFILE.db MG7.bam-ANVIO_PROFILE/PROFILE.db -o SAMPLES-MERGED -c contigs.db [22 Apr 19 03:05:35] clustering_performed .........................: False [22 Apr 19 03:19:05] WARNING =============================================== Codon frequencies were not profiled, hence, these tables will be empty in the merged profile database. [22 Apr 19 03:20:31] * Anvi'o hierarchical clustering of contigs... [22 Apr 19 03:20:31] * Automatic binning with CONCOCT... [25 Apr 19 13:47:30] * Additional data and layer orders... [25 Apr 19 13:48:24] Layer orders added =============================================== [25 Apr 19 13:48:24] * std_coverage [25 Apr 19 13:48:24] * mean_coverage [25 Apr 19 13:48:24] * mean_coverage_Q2Q3 [25 Apr 19 13:48:24] * max_normalized_ratio [25 Apr 19 13:48:24] * relative_abundance [25 Apr 19 13:48:24] * detection [25 Apr 19 13:48:24] * abundance [25 Apr 19 13:48:24] * variability [25 Apr 19 13:48:24] Data groups added =============================================== [25 Apr 19 13:48:24] * default (w/2 items) [25 Apr 19 13:48:25] * Happy [25 Apr 19 13:48:25] Bye.