Bismark report for: /gscratch/scrubbed/samwhite/outputs/20190312_cvir_gonad_bismark/cvir_bsseq_all_pe_R1.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20190312_cvir_gonad_bismark/cvir_bsseq_all_pe_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_virginica/genomes/GCF_002022765.2_C_virginica-3.0/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 275914272 Number of paired-end alignments with a unique best hit: 90400225 Mapping efficiency: 32.8% Sequence pairs with no alignments under any condition: 142295313 Sequence pairs did not map uniquely: 43218734 Sequence pairs which were discarded because genomic sequence could not be extracted: 9 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 23103068 ((converted) top strand) GA/CT/CT: 22071301 (complementary to (converted) top strand) GA/CT/GA: 22087710 (complementary to (converted) bottom strand) CT/GA/GA: 23138137 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2447811202 Total methylated C's in CpG context: 252963945 Total methylated C's in CHG context: 10974793 Total methylated C's in CHH context: 38264164 Total methylated C's in Unknown context: 1418685 Total unmethylated C's in CpG context: 82162796 Total unmethylated C's in CHG context: 546133588 Total unmethylated C's in CHH context: 1517311916 Total unmethylated C's in Unknown context: 8077086 C methylated in CpG context: 75.5% C methylated in CHG context: 2.0% C methylated in CHH context: 2.5% C methylated in unknown context (CN or CHN): 14.9% Bismark completed in 2d 20h 32m 48s