Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v073/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 248421 Mapping efficiency: 49.7% Sequences with no alignments under any condition: 173607 Sequences did not map uniquely: 77972 Sequences which were discarded because genomic sequence could not be extracted: 1867 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 124253 ((converted) top strand) CT/GA: 122301 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 3932963 Total methylated C's in CpG context: 133375 Total methylated C's in CHG context: 20534 Total methylated C's in CHH context: 36870 Total methylated C's in Unknown context: 773 Total unmethylated C's in CpG context: 438843 Total unmethylated C's in CHG context: 798954 Total unmethylated C's in CHH context: 2504387 Total unmethylated C's in Unknown context: 9063 C methylated in CpG context: 23.3% C methylated in CHG context: 2.5% C methylated in CHH context: 1.5% C methylated in Unknown context (CN or CHN): 7.9% Bismark completed in 0d 0h 3m 27s