Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v071/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 5000000 Number of alignments with a unique best hit from the different alignments: 2697574 Mapping efficiency: 54.0% Sequences with no alignments under any condition: 1444259 Sequences did not map uniquely: 858167 Sequences which were discarded because genomic sequence could not be extracted: 18994 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1352788 ((converted) top strand) CT/GA: 1325792 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 42988967 Total methylated C's in CpG context: 1419247 Total methylated C's in CHG context: 225859 Total methylated C's in CHH context: 388535 Total methylated C's in Unknown context: 8009 Total unmethylated C's in CpG context: 4809714 Total unmethylated C's in CHG context: 8806740 Total unmethylated C's in CHH context: 27338872 Total unmethylated C's in Unknown context: 104372 C methylated in CpG context: 22.8% C methylated in CHG context: 2.5% C methylated in CHH context: 1.4% C methylated in Unknown context (CN or CHN): 7.1% Bismark completed in 0d 0h 33m 29s