Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v071/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 269073 Mapping efficiency: 53.8% Sequences with no alignments under any condition: 145406 Sequences did not map uniquely: 85521 Sequences which were discarded because genomic sequence could not be extracted: 1830 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 134995 ((converted) top strand) CT/GA: 132248 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 4292056 Total methylated C's in CpG context: 141617 Total methylated C's in CHG context: 22474 Total methylated C's in CHH context: 39349 Total methylated C's in Unknown context: 828 Total unmethylated C's in CpG context: 480543 Total unmethylated C's in CHG context: 880344 Total unmethylated C's in CHH context: 2727729 Total unmethylated C's in Unknown context: 10369 C methylated in CpG context: 22.8% C methylated in CHG context: 2.5% C methylated in CHH context: 1.4% C methylated in Unknown context (CN or CHN): 7.4% Bismark completed in 0d 0h 3m 47s