Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v071/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10000000 Number of alignments with a unique best hit from the different alignments: 5392088 Mapping efficiency: 53.9% Sequences with no alignments under any condition: 2893485 Sequences did not map uniquely: 1714427 Sequences which were discarded because genomic sequence could not be extracted: 37064 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2705305 ((converted) top strand) CT/GA: 2649719 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 85972609 Total methylated C's in CpG context: 2840905 Total methylated C's in CHG context: 452489 Total methylated C's in CHH context: 778216 Total methylated C's in Unknown context: 15821 Total unmethylated C's in CpG context: 9613477 Total unmethylated C's in CHG context: 17605608 Total unmethylated C's in CHH context: 54681914 Total unmethylated C's in Unknown context: 208382 C methylated in CpG context: 22.8% C methylated in CHG context: 2.5% C methylated in CHH context: 1.4% C methylated in Unknown context (CN or CHN): 7.1% Bismark completed in 0d 1h 7m 3s