Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v070/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 280278 Mapping efficiency: 56.1% Sequences with no alignments under any condition: 100581 Sequences did not map uniquely: 119141 Sequences which were discarded because genomic sequence could not be extracted: 580 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 139544 ((converted) top strand) CT/GA: 140154 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 4524107 Total methylated C's in CpG context: 145463 Total methylated C's in CHG context: 23550 Total methylated C's in CHH context: 41977 Total methylated C's in Unknown context: 887 Total unmethylated C's in CpG context: 524273 Total unmethylated C's in CHG context: 934834 Total unmethylated C's in CHH context: 2854010 Total unmethylated C's in Unknown context: 10126 C methylated in CpG context: 21.7% C methylated in CHG context: 2.5% C methylated in CHH context: 1.4% C methylated in Unknown context (CN or CHN): 8.1% Bismark completed in 0d 0h 4m 19s