Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/olur_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/O_lurida/genomes/Olurida_v081/oly_v081_bismark_genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 5000000 Number of alignments with a unique best hit from the different alignments: 2715238 Mapping efficiency: 54.3% Sequences with no alignments under any condition: 915387 Sequences did not map uniquely: 1369375 Sequences which were discarded because genomic sequence could not be extracted: 2005 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1351525 ((converted) top strand) CT/GA: 1361708 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 23890177 Total methylated C's in CpG context: 1220797 Total methylated C's in CHG context: 139846 Total methylated C's in CHH context: 273458 Total methylated C's in Unknown context: 7542 Total unmethylated C's in CpG context: 2796260 Total unmethylated C's in CHG context: 4903686 Total unmethylated C's in CHH context: 14556130 Total unmethylated C's in Unknown context: 88121 C methylated in CpG context: 30.4% C methylated in CHG context: 2.8% C methylated in CHH context: 1.8% C methylated in Unknown context (CN or CHN): 7.9% Bismark completed in 0d 0h 25m 58s