Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/olur_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/O_lurida/genomes/Olurida_v081/oly_v081_bismark_genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 2000000 Number of alignments with a unique best hit from the different alignments: 1087693 Mapping efficiency: 54.4% Sequences with no alignments under any condition: 365638 Sequences did not map uniquely: 546669 Sequences which were discarded because genomic sequence could not be extracted: 792 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 541869 ((converted) top strand) CT/GA: 545032 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 9569425 Total methylated C's in CpG context: 488699 Total methylated C's in CHG context: 55998 Total methylated C's in CHH context: 109662 Total methylated C's in Unknown context: 2939 Total unmethylated C's in CpG context: 1119419 Total unmethylated C's in CHG context: 1963491 Total unmethylated C's in CHH context: 5832156 Total unmethylated C's in Unknown context: 34902 C methylated in CpG context: 30.4% C methylated in CHG context: 2.8% C methylated in CHH context: 1.8% C methylated in Unknown context (CN or CHN): 7.8% Bismark completed in 0d 0h 10m 49s