Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz and /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v070/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 5000000 Number of paired-end alignments with a unique best hit: 2781620 Mapping efficiency: 55.6% Sequence pairs with no alignments under any condition: 1380229 Sequence pairs did not map uniquely: 838151 Sequence pairs which were discarded because genomic sequence could not be extracted: 3301 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1383875 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 1394444 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 89926108 Total methylated C's in CpG context: 2751759 Total methylated C's in CHG context: 396227 Total methylated C's in CHH context: 699376 Total methylated C's in Unknown context: 11850 Total unmethylated C's in CpG context: 10353939 Total unmethylated C's in CHG context: 18822996 Total unmethylated C's in CHH context: 56901811 Total unmethylated C's in Unknown context: 172910 C methylated in CpG context: 21.0% C methylated in CHG context: 2.1% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 6.4% Bismark completed in 0d 1h 5m 50s