Bismark report for: /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R1.fastq.gz and /gscratch/scrubbed/samwhite/data/P_generosa/BSeq/rrbs/pgen_bsseq_all_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/P_generosa/Pgenerosa_v070/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 555406 Mapping efficiency: 55.5% Sequence pairs with no alignments under any condition: 277284 Sequence pairs did not map uniquely: 167310 Sequence pairs which were discarded because genomic sequence could not be extracted: 654 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 276549 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 278203 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 17976868 Total methylated C's in CpG context: 550216 Total methylated C's in CHG context: 78782 Total methylated C's in CHH context: 141704 Total methylated C's in Unknown context: 2523 Total unmethylated C's in CpG context: 2073151 Total unmethylated C's in CHG context: 3760624 Total unmethylated C's in CHH context: 11372391 Total unmethylated C's in Unknown context: 34338 C methylated in CpG context: 21.0% C methylated in CHG context: 2.1% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 6.8% Bismark completed in 0d 0h 13m 52s