Bismark report for: /gscratch/scrubbed/samwhite/data/C_virginica/BSseq/cvir_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_virginica/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 93015 Mapping efficiency: 18.6% Sequences with no alignments under any condition: 357549 Sequences did not map uniquely: 49436 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 46787 ((converted) top strand) CT/GA: 46228 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1400833 Total methylated C's in CpG context: 147581 Total methylated C's in CHG context: 12397 Total methylated C's in CHH context: 93529 Total methylated C's in Unknown context: 593 Total unmethylated C's in CpG context: 47260 Total unmethylated C's in CHG context: 279300 Total unmethylated C's in CHH context: 820766 Total unmethylated C's in Unknown context: 2972 C methylated in CpG context: 75.7% C methylated in CHG context: 4.2% C methylated in CHH context: 10.2% C methylated in Unknown context (CN or CHN): 16.6% Bismark completed in 0d 0h 1m 34s