Bismark report for: /gscratch/scrubbed/samwhite/data/C_virginica/BSseq/cvir_bsseq_all_R1.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_virginica/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 2000000 Number of alignments with a unique best hit from the different alignments: 390488 Mapping efficiency: 19.5% Sequences with no alignments under any condition: 1400838 Sequences did not map uniquely: 208674 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 195707 ((converted) top strand) CT/GA: 194781 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 5821219 Total methylated C's in CpG context: 608878 Total methylated C's in CHG context: 43463 Total methylated C's in CHH context: 335814 Total methylated C's in Unknown context: 2309 Total unmethylated C's in CpG context: 197333 Total unmethylated C's in CHG context: 1174311 Total unmethylated C's in CHH context: 3461420 Total unmethylated C's in Unknown context: 13237 C methylated in CpG context: 75.5% C methylated in CHG context: 3.6% C methylated in CHH context: 8.8% C methylated in Unknown context (CN or CHN): 14.9% Bismark completed in 0d 0h 5m 21s