Bismark report for: /gscratch/scrubbed/samwhite/data/C_virginica/BSseq/cvir_bsseq_all_pe_R1.fastq.gz and /gscratch/scrubbed/samwhite/data/C_virginica/BSseq/cvir_bsseq_all_pe_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sam/data/C_virginica/genomes/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 500000 Number of paired-end alignments with a unique best hit: 67032 Mapping efficiency: 13.4% Sequence pairs with no alignments under any condition: 409150 Sequence pairs did not map uniquely: 23818 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 33494 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 33538 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1981660 Total methylated C's in CpG context: 211845 Total methylated C's in CHG context: 8665 Total methylated C's in CHH context: 50921 Total methylated C's in Unknown context: 569 Total unmethylated C's in CpG context: 75912 Total unmethylated C's in CHG context: 429847 Total unmethylated C's in CHH context: 1204470 Total unmethylated C's in Unknown context: 6420 C methylated in CpG context: 73.6% C methylated in CHG context: 2.0% C methylated in CHH context: 4.1% C methylated in unknown context (CN or CHN): 8.1% Bismark completed in 0d 0h 2m 5s